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20 g. Yeast extract. 5 g. NaCl. 0.5 g. SOC medium is identical to SOB medium, except that it contains 20 m m glucose. To prepare SOB medium, combine the above ingredients and shake until the solutes have dissolved. Add 10 mL of a 250 m m solution of KCl.
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9. ( Super Optimal broth with Catabolite Repression) SOC media contains glucose whereas Luria Broth (LB) doesn't. SOB also has a more delicate salt balance. Naturally the glucose will help the cells recover and grow faster after the heat shock. Your transformation efficiency should improve since less cells will die.
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S.O.C. medium has the same formulation as the rich S.O.B. (Super Optimal Broth) medium with additionally added 10 mM MgCl 2 and 20 mM glucose (as published by Hanahan in 1983). S.O.C. Medium is supplied as 10 x 10 mL bottles of ready-to-use liquid medium. Each lot of S.O.C. Medium is tested to ensure conformance with the most current approved.
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SOC Medium, shorthand for Super Optimal broth with Catabolite repression, is a nutritionally rich bacterial culture medium. The recipe is differs slightly with LB broth, containing 20 g/L tryptone, 5 g/L yeast extract, and 0.5 g/L sodium chloride. A defining ingredient is 20 mM glucose, which must be added separately after the media has cooled.
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Add SOC to freshly transformed E. coli cells and incubate for up to 1 hour before plating on selective media. 1X SOC Outgrowth Medium: 2% Vegetable Peptone 0.5% Yeast Extract 10 mM NaCl 2.5 mM KCl 10 mM MgCl2 10 mM MgSO4 20 mM Glucose. This product is related to the following categories: Competent Cells Buffers & Diluents Products, Buffers Products
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The following recipe is standardized to 1 L 1. Prepare stock solutions of glucose, MgCl2 and MgSO4 and filter sterilize them a. Glucose 2 M: 18 g glucose in 90 mL of ddH2O, fill up to 100 mL b. MgCl2 2 M: 19 g MgCl2 in 90 mL of ddH2O, fill up to 100 mL c. MgSO4 2 M: 49.3 g MgSO4 x 7 H2O in 90 mL of ddH2O, fill up to 100 mL d.
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Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli.This nutrient-rich microbial broth contains peptides, amino acids, water soluble vitamins and glucose in a low-salt formulation. It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used LB medium (lysogeny broth).
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Table Of Contents Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for growing.
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Step 3: Cool down the medium to room temperature. Add 2.5 ml of 1M KCl and mix. Adjust the pH 7.0 with 5N NaOH (~0.2 ml). Note:The SOB medium is not very highly buffered. The pH of the medium drops, as the growing culture reaches the near saturation phase. Step 4: Adjust the volume to 960 ml with deionized / Milli-Q water.
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Just before use, add 5 mL of a sterile solution of 2 m MgCl 2. (This solution is made by dissolving 19 g of MgCl 2 in 90 mL of deionized H 2 O. Adjust the volume of the solution to 100 mL with deionized H 2 O and sterilize by autoclaving for 20 min at 15 psi [1.05 kg/cm 2] on liquid cycle.) For solid medium, please see the recipe entitled.
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Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the.
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Recipe. SOC Medium for E. coli. Reagent Final concentration; Tryptone: 2% (w/v) Yeast extract: 0.5% (w/v) NaCl: 10 m m: KCl: 2.5 m m: MgCl 2: 10 m m: Glucose: 20 m m: Prepare a solution containing the first four reagents, sterilize at 121 °C, and then add sterile MgCl 2 and glucose.
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2.5 mM KCl. 10 mM MgCl2. 10 mM MgSO4. 20 mM Glucose*. *Note: add Glucose after autoclaving the solution with the remaining ingredients, and letting it cool down. Sterilize the final solution by passing it through a 0.2 µm filter. SOC medium can be stored at room temperature and is stable for several years. icon_0011_idea-s.
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Soc medium preparation: Used in bacterial transformation. Ingredients for SOB: 0.5% (w/v) yeast extract 2% (w/v) tryptone 10 mM NaCl 2.5 mM KCl 20 mM MgSO 4 Per liter: 5 g yeast extract 20 g tryptone 0.584 g NaCl 0.186 g KCl 2.4 g MgSO 4 Note: Some formulations of SOB use 10 mM MgCl 2 and 10 mM MgSO 4
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SOB (Super Optimal Broth) medium is a nutrient-rich microbial growth medium, designed by Douglas Hanahan in 1983. SOB or its glucose-supplemented version SOC is often used during transformation of E. coli competent cells with plasmids (added in transformed cells after heat shock). It results in better survival and higher transformation efficiency as compared to regular LB medium. It contains 2.